Welcome to the Confocal Microscope Facility

 
Triple stain 2
The Nikon Eclipse Ti confocal laser scanning microscope is equipped with four laser light sources, differential interference contrast and temperature controlled stage and is used for research and teaching purposes.  Several departments at Amherst College have access to the facility which resides in McGuire Life Sciences.

Please contact Lori Nichols at 413-542-2081 with any questions.

 

View work from labs via navigation buttons to your left under "Confocal Microscope".

Reference Materials for Students:

 
 CellBASE for Research resources, Forums and Blogs, Model Organisms, Methods, Protocols, Tutorials, Simulation and Animation and related resources
 
Molecular Expressions for Photo Galleries, Imaging Tutorials, Videos, LSM and Simulations
 
Nikon Microscopy U for general microscopy education, Tutorials, Galleries and Downloads
 
Olympus Microscopy Resource Center for Physics, Anatomy and Tecnique
 
 University of Arizona-Cellular Imaging Facility for General and Historical information on Confocal Theory and Operation, also Optics and Hardware
 
Purdue University has several Powerpoint Lecture Slides on different aspects of microscopy including confocal.
ZEISS has tutorials and articles on Education in Microscopy and Digital Imaging
 

Principles of Confocal MicroscopyPrinciples of Confocal Microscopy by Karen Lovely-Leach

 

Student Work:

 PLC gamma in Fertilized Sea Urchin Eggs by Michelle Huynh '11

PLC gammaPolyspermyTriple stain 2Triple stain
          Telophase               Polyspermy           Triple staining             Enlarged
 
          Green - PLC gamma       Blue - DNA       Red - Endoplasmic Reticulum
 

Z-Series ER in L. pictus Egg, by Burr Fong '08

Slice 1Slice 2Slice 3Slice 4Slice 5
Slice 6Slice 7Slice 8Slice 9NE and Nucleus
 

Click on the movie file to view: z-series movie  of Endoplasmic Reticulum in the sea urchin egg post fertilization.

ER movieER movie

 

 

Facility Users:

 The microscope is a vital tool for research.

 4  summer research students and one technician.

Poccia Lab
From Left to Right in the Group Photo:
Michelle, Zina, Julie, Ashley, and Professor Poccia seated ...who's wearing the captain's hat?
 Michelle
 
Michelle just heard that the urchin - she's got eggs!

Zina

Zina is going after those precious eggs.
   image
Professor Poccia is our faculty expert. 

 

Last summer 6 summer research students and one technician

 

Confocal Crew

Confocal Consulting

Left to Right:  Crystal, Julie, Ben, Darienne, Jen

We weren't able to "capture" Neil

 
It's not uncommon to see students consulting with one another