Welcome to the Confocal Microscope Facility

Triple stain 2
The Nikon Eclipse Ti confocal laser scanning microscope is equipped with four laser light sources, differential interference contrast and temperature controlled stage and is used for research and teaching purposes.  Several departments at Amherst College have access to the facility which resides in McGuire Life Sciences.

Please contact Lori Nichols at 413-542-2081 with any questions.


View work from labs via navigation buttons to your left under "Confocal Microscope".

Reference Materials for Students:

 CellBASE for Research resources, Forums and Blogs, Model Organisms, Methods, Protocols, Tutorials, Simulation and Animation and related resources
Molecular Expressions for Photo Galleries, Imaging Tutorials, Videos, LSM and Simulations
Nikon Microscopy U for general microscopy education, Tutorials, Galleries and Downloads
Olympus Microscopy Resource Center for Physics, Anatomy and Tecnique
 University of Arizona-Cellular Imaging Facility for General and Historical information on Confocal Theory and Operation, also Optics and Hardware
Purdue University has several Powerpoint Lecture Slides on different aspects of microscopy including confocal.
ZEISS has tutorials and articles on Education in Microscopy and Digital Imaging

Principles of Confocal MicroscopyPrinciples of Confocal Microscopy by Karen Lovely-Leach


Student Work:

 PLC gamma in Fertilized Sea Urchin Eggs by Michelle Huynh '11

PLC gammaPolyspermyTriple stain 2Triple stain
          Telophase               Polyspermy           Triple staining             Enlarged
          Green - PLC gamma       Blue - DNA       Red - Endoplasmic Reticulum

Z-Series ER in L. pictus Egg, by Burr Fong '08

Slice 1Slice 2Slice 3Slice 4Slice 5
Slice 6Slice 7Slice 8Slice 9NE and Nucleus

Click on the movie file to view: z-series movie  of Endoplasmic Reticulum in the sea urchin egg post fertilization.

ER movieER movie



Facility Users:

 The microscope is a vital tool for research.

 4  summer research students and one technician.

Poccia Lab
From Left to Right in the Group Photo:
Michelle, Zina, Julie, Ashley, and Professor Poccia seated ...who's wearing the captain's hat?
Michelle just heard that the urchin - she's got eggs!


Zina is going after those precious eggs.
Professor Poccia is our faculty expert. 


Last summer 6 summer research students and one technician


Confocal Crew

Confocal Consulting

Left to Right:  Crystal, Julie, Ben, Darienne, Jen

We weren't able to "capture" Neil

It's not uncommon to see students consulting with one another

McGuire Life Sciences Building