ACID BASE LAB EXPT 7

Submitted by Patricia B. O'Hara on Wednesday, 11/18/2009, at 5:04 PM

Virtual DNA Microarray Lab

Submitted by Caroline E. Goutte on Tuesday, 11/3/2009, at 10:12 PM

DNA microarray analysis allows you to compare different populations of RNA transcripts.

For example, you might want to compare the transcription levels of  gene x in muscle cells versus blood cells, but imagine being able to ask the same question for every gene in the human genome - ie. how do the transcript levels of all the human genes compare in muscle cells versus blood cells.  This type of analysis has been enlightening in terms of teaching us exactly what makes a muscle cell versus a blood cell, but it has been particularly powerful as a research tool for cancer biology where it is not as easy to tell apart a normal versus a cancerous cell - especially in the early stages.

The Genetic Science Learning Center at the University of Utah has put together a virtual DNA microarray lab to allow you to try this technique out.

http://learn.genetics.utah.edu/content/labs/microarray/

web sites for beta globin lab

Submitted by Caroline E. Goutte on Wednesday, 10/21/2009, at 2:00 PM

beta globin lab 10-21-09

Submitted by Caroline E. Goutte on Wednesday, 10/21/2009, at 1:58 PM

Hemoglobin Structure Lab word

Submitted by Patricia B. O'Hara on Wednesday, 10/21/2009, at 12:58 PM

Hemoglobin Structure Lab pdf

Submitted by Patricia B. O'Hara on Wednesday, 10/21/2009, at 12:58 PM

Blood Glucose Lab

Submitted by Patricia B. O'Hara on Saturday, 10/3/2009, at 6:09 PM
I've processed data for BREAD as an example, but not YOGURT. Things got a bit complicated since the bread pieces were different masses, and we had to take that into account. Since GI is measured for 100 g, I NORMALIZED all the ratios to 100 g. (If you don't know what that means, I'll explain Monday) You should do calculations for YOGURT, where all the masses were the same (170 g) but still, that needs to be normalized to 100 g to compare with GI. Once you've got that all squared away, you should plot both HIGH GI BREAD vs time and LOW GI Yogurt vs time PREFERABLY on the same set of axes. We'll go over the calculations for Q6 in class Monday if you don't understand them. It's not so straightforward.
 

Taking Notes