Methods in Neuroscience

Transgenic Techniques II
Explanation of pronuclear injection technique from Zhao at UCSD
Transgenic Techniques I
Nice explanation from Dr. Campbell of Davidson Biology on how to make Knockouts.
Receptor binding tutorial
Well presented interactive tutorial by Jean Deupree and Matthew Lurz at the University of Nebraska Medical Center. Includes a complete derivation of the Scatchard (also called Rosenthal) analysis that allows us to find Bmax and KD for a given receptor. (The tutorial runs under Internet Explorer but not Netscape or Firefox.)
How to make antibodies
Here is a nice simple page from a retired professor's onine biology text.
image

Methods in Neuroscience

Transgenic Techniques II
Explanation of pronuclear injection technique from Zhao at UCSD
Transgenic Techniques I
Nice explanation from Dr. Campbell of Davidson Biology on how to make Knockouts.
Receptor binding tutorial
Well presented interactive tutorial by Jean Deupree and Matthew Lurz at the University of Nebraska Medical Center. Includes a complete derivation of the Scatchard (also called Rosenthal) analysis that allows us to find Bmax and KD for a given receptor. (The tutorial runs under Internet Explorer but not Netscape or Firefox.)
How to make antibodies
Here is a nice simple page from a retired professor's onine biology text.
image