The emerging field of biosensors based on two-dimensional (2D) materials offers a pathway to new opportunities in microbioanalytics, highlighting next-generation tools for point-of-care diagnostic, health care, and environmental monitoring. My talk will focus on the unique transduction properties of 2D materials and the downstream biomolecular-sensor applications with unprecedented high sensitivity and efficiency in power, size and cost. I will discuss the self-transducibility of 2D materials at the interface to biofluids; the methodologies I developed to fabricate, functionalize and implement small-scale (~ ????m) 2D-based biosensing devices; and label-free detection of various biomarkers (nucleic acid oligomers, proteinaceous antigens, opioid-neuropeptides, pH) with high sensitivity, e.g. attomolar for DNA, by using the devices. I will conclude by introducing a handheld multiplexed water-quality monitoring robot enabled by graphene aptasensors.